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KMID : 0922220110110010001
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Journal of the Korean Musculoskeletal Transplantation Society
2011 Volume.11 No. 1 p.1 ~ p.7
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Effectiveness of Microbiological and Fungal Cultures of Deep Frozen Allograft just before Implantation
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Seo Hyuk-Jun
Lee Kyung-Jae
Min Byung-Woo
Bae Ki-Cheor
Son Eun-Seok
Jeong Kwang-Yeung
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Abstract
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Purpose: The purpose of this study was to assess the clinical effectiveness of microbiological and fungal culture of deep frozen allograft just before implantation.
Materials and Methods: Between November 2007 and April 2010, 76 femoral heads were obtained and stored at our hospital. Among them 69 allografts were transplanted and evaluated in this study. All of the procured bones were resected femoral heads during primary total hip arthroplasty or primary bipolar hemi-arthroplasty and there was no donor who had inflammatory disease. Bone grafts were performed during revision or re-revision total hip arthroplasty in 51 patients and average. 1.4 (range, 1~3) allografts were used at each operation. Before implantation, swab cultures for bacteria and fungus were performed. After Bone graft, we evaluated clinical evidence of infection and radiological results during regular follow-up period.
Results: Among 69 implanted allografts, 4 positive-cultured femoral heads (5.8%) were detected and all were positive-fungal culture. Four positive-cultured allografts were implanted in 4 patients and deep infection occurred in two patients. None of them showed the same microorganism caused the contamination and the subsequent infection. There was also one deep infection in patient who received negative-cultured allograft.
Conslusion: There was relatively high positive-cultured rate just before implantation of allografts. But positive-cultured allograft did not cause deep infection with the same micro-organism in clinical setting. We suggest that positive cultured allograft just before implantation probably represent contamination and that culture before implantation has little effectiveness clinically.
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KEYWORD
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Allograft, Culture, Contamination, Infection, Implantation
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